9CRN

Crystal structure of Streptococcus pyogenes TglA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	51.272, 88.334, 94.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.020 - 1.960
R-factor	0.1906
Rwork	0.189
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.675
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.990
High resolution limit [Å]	1.960	1.960
Number of reflections	31441	1508
<I/σ(I)>	10.58
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.970	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM Bis-Tris (pH 6.5), 200 mM sodium chloride, 15% PEG 3350