9COZ
Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella philomiragia (Orthorhombic P Form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-07-14 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.272, 62.107, 79.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.930 - 1.700 |
R-factor | 0.1865 |
Rwork | 0.184 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.014 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_5383: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.930 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.107 | 2.052 |
Rmeas | 0.112 | 2.132 |
Rpim | 0.031 | 0.575 |
Total number of observations | 258287 | 13912 |
Number of reflections | 19913 | 1021 |
<I/σ(I)> | 14.1 | 1.5 |
Completeness [%] | 100.0 | |
Redundancy | 13 | 13.6 |
CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Morpheus C12: 20% (v/v) Ethylene glycol; 10 % (w/v) PEG 8000; 0.02M D-Glucose; 0.05M Sodium HEPES; 0.05M MOPS (acid) pH 7.5; 0.02M D-Mannose; 0.02M D-Galactose; 0.02M L-Fucose; 0.02M D-Xylose; 0.02M N-Acetyl-D-Glucosamine; 0.2% w/v Lidocaine hydrochloride monohydrate, 0.2% w/v Procaine hydrochloride, 0.2%w/v Proparacaine hydrochloride, 0.2% w/v tetracaine hydrochloride . FrphA.20731.a.VL2.PB00125 at 13.9 mg/mL. plate 14180 well C12 drop2, Puck: PSL0901, Cryo: Direct |