9CIW
Penguinpox cGAMP PDE H72A mutant in complex with 2'3'-cGAMP
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2024-06-21 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.967, 64.963, 196.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.380 - 2.400 |
R-factor | 0.1978 |
Rwork | 0.195 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.001 |
RMSD bond angle | 0.405 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.390 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.016 | 0.282 |
Number of reflections | 34185 | 2580 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 99.6 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 200 mM magnesium formate 24% (w/v) PEG 3350 |