9CIW
Penguinpox cGAMP PDE H72A mutant in complex with 2'3'-cGAMP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-06-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.967, 64.963, 196.424 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.380 - 2.400 |
| R-factor | 0.1978 |
| Rwork | 0.195 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.405 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.390 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.016 | 0.282 |
| Number of reflections | 34185 | 2580 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.6 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 200 mM magnesium formate 24% (w/v) PEG 3350 |
