9CIS
Crystal structure of the pyrophosphate-dependent phosphofructokinase from Candidatus Prometheoarchaeum syntrophicum with fructose 6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-30 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.95366 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.210, 71.866, 136.530 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.350 |
| R-factor | 0.2471 |
| Rwork | 0.244 |
| R-free | 0.31440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.290 | 8.630 |
| Rmeas | 0.300 | 8.940 |
| Rpim | 0.080 | 2.350 |
| Number of reflections | 69107 | 4391 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 1 M MMT pH 9.0, 25 % PEG 1500 |
