9CH2
Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA-L63D) heteromeric complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.270, 92.270, 162.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.830 - 2.350 |
| R-factor | 0.19263 |
| Rwork | 0.190 |
| R-free | 0.24013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.565 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.069 | 1.339 |
| Number of reflections | 33473 | 3932 |
| <I/σ(I)> | 36.32 | |
| Completeness [%] | 98.4 | |
| Redundancy | 12.05 | |
| CC(1/2) | 1.000 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 240 mM sodium malonate pH 5.5 with 8% PEG 3350 |
