9CF7

Germline-targeting HIV-1 gp120 engineered outer domain eODgt8 in complex with Fab eOD-PL01.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 64 2 2
Unit cell lengths	166.767, 166.767, 100.693
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.550 - 3.550
Rwork	0.265
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.550	3.890
High resolution limit [Å]	3.550	3.550
Rmerge	0.260	3.100
Rmeas	0.274	3.250
Rpim	0.087	1.000
Number of reflections	10327	2421
<I/σ(I)>	9.1	1
Completeness [%]	99.4	99.9
Redundancy	9.5	9.9
CC(1/2)	0.997	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M potassium dihydrogen phosphate, 20% Peg3350