9CD2

Structure of E. coli ZDHYS365 multi-ubiquitin domain protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-31
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 32 2 1
Unit cell lengths	117.538, 117.538, 104.444
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.460 - 1.870
R-factor	0.1832
Rwork	0.182
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.398
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.460	1.910
High resolution limit [Å]	1.870	1.870
Rmerge	0.093	2.232
Rpim	0.032	0.978
Number of reflections	69110	4378
<I/σ(I)>	16.1	0.8
Completeness [%]	100.0	100
Redundancy	9.5	6.1
CC(1/2)	0.999	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	1 M LiCl, 0.1 M sodium acetate, and 30% PEG 6000