9C9R
Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain, F1090C/G1326S variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.111, 52.714, 136.891 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 45.710 - 2.320 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.710 | 2.320 |
| High resolution limit [Å] | 2.316 | 2.316 |
| Number of reflections | 95403 | 49637 |
| <I/σ(I)> | 6.41 | 0.96 |
| Completeness [%] | 99.3 | |
| Redundancy | 8.5 | |
| CC(1/2) | 0.992 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES pH 7.5, 15% v/v ethylene glycol and 10% v/v PEG 8000 |
