9C9Q
Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.431, 52.003, 136.427 |
Unit cell angles | 90.00, 104.14, 90.00 |
Refinement procedure
Resolution | 35.530 - 2.000 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.789 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.530 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 68536 | 3881 |
<I/σ(I)> | 19.2 | 0.72 |
Completeness [%] | 98.1 | |
Redundancy | 3.3 | |
CC(1/2) | 0.993 | 0.286 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M ammonium sulfate, 100 mM sodium cacodylate pH 6.5, 30% w/v polyethylene glycol (PEG) 8000. |