9C9I
Structure of the TSC1:WIPI3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 41 |
| Unit cell lengths | 94.241, 94.241, 199.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.120 - 3.180 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.427 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 3.370 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Number of reflections | 29039 | 4425 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 98.9 | 93.4 |
| Redundancy | 13.9 | |
| CC(1/2) | 0.997 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M ammonium tartrate, 20 % PEG3350, and 0.25 M sodium acetate. |
