9C9I

Structure of the TSC1:WIPI3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 41
Unit cell lengths	94.241, 94.241, 199.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.120 - 3.180
R-factor	0.2005
Rwork	0.198
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.427
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.120	3.370
High resolution limit [Å]	3.170	3.170
Number of reflections	29039	4425
<I/σ(I)>	11.1
Completeness [%]	98.9	93.4
Redundancy	13.9
CC(1/2)	0.997	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M ammonium tartrate, 20 % PEG3350, and 0.25 M sodium acetate.