9C6L

Yasminevirus c12orf29, a 5' to 3' RNA ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	104.897, 84.221, 115.240
Unit cell angles	90.00, 106.88, 90.00

Refinement procedure

Resolution	46.410 - 2.700
R-factor	0.2341
Rwork	0.232
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.543
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.190	0.110	1.229
Rmeas	0.200	0.116	1.328
Rpim	0.061	0.035	0.488
Total number of observations	550364
Number of reflections	55600	2925	2382
<I/σ(I)>	4.7
Completeness [%]	98.0	99	85
Redundancy	9.9	10.9	6
CC(1/2)	0.963	0.974	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.85 M sodium malonate, 50 mM NaCl, 0.1 M Bis-Tris, 20% ethylene glycol