9C66
Structure of the Mena EVH1 domain bound to the polyproline segment of PTP1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.654, 49.072, 54.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.400 |
| R-factor | 0.1877 |
| Rwork | 0.187 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.095 | 0.577 |
| Rmeas | 0.100 | 0.606 |
| Rpim | 0.029 | 0.180 |
| Total number of observations | 259048 | 12450 |
| Number of reflections | 21372 | 1040 |
| <I/σ(I)> | 18.8 | 6.3 |
| Completeness [%] | 99.6 | |
| Redundancy | 12.1 | 12 |
| CC(1/2) | 0.998 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 2.0 M Ammonium sulfate and 0.1 M Bis-Tris |
