9C4S

Menin mutant G331R in complex with MLL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	48.695, 80.012, 124.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.290 - 1.540
Rwork	0.172
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.905
Data reduction software	XDS
Data scaling software	Aimless (0.7.15)
Phasing software	MOLREP (11.0 / 22.07.2010/)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.290	1.620
High resolution limit [Å]	1.540	1.540
Rmerge	0.056	0.569
Number of reflections	72717	10489
<I/σ(I)>	16.3	2.5
Completeness [%]	100.0	100
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350