9C1A

Crystal structure of GDP-bound human M-RAS protein in crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 6 2 2
Unit cell lengths	108.653, 108.653, 65.759
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.330 - 1.960
R-factor	0.1747
Rwork	0.172
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.876
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.330	2.030
High resolution limit [Å]	1.960	1.960
Rmeas	0.202	1.732
Rpim	0.032	0.293
Number of reflections	16016	1643
<I/σ(I)>	19.82	2.5
Completeness [%]	94.0	99.88
Redundancy	38.3
CC(1/2)	1.000	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2M CaCl2, 20% PEG3350