9C11
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2023-11-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.183, 53.201, 107.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.760 - 1.950 |
| R-factor | 0.21313 |
| Rwork | 0.211 |
| R-free | 0.26094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.764 |
| Data reduction software | CrysalisPro (1.171.42.95a) |
| Data scaling software | CrysalisPro (1.171.42.95a) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.210 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.015 | 0.193 |
| Number of reflections | 22934 | 2297 |
| <I/σ(I)> | 104.39 | 5.88 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 9.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 28% MPD 25mM K Phosphate |
