9C0O

Crystal structure of DmCfp1 PHD finger bound to H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.270, 43.120, 47.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.950 - 1.530
R-factor	0.158
Rwork	0.156
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.713
Data reduction software	xia2
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.950	1.585
High resolution limit [Å]	1.530	1.530
Rmerge	0.134	1.372
Rmeas	0.140	1.425
Rpim	0.039	0.381
Number of reflections	10748	1046
<I/σ(I)>	11.6	2.1
Completeness [%]	99.5	98.87
Redundancy	12.8	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	15% PEG3350, 0.01M Tris pH8.5, 0.2M Ammonium sulfate