9BQ4
YTP-E E148D, a weakly yellow thermostable protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.784, 137.777, 74.556 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 20.320 - 3.000 |
| R-factor | 0.2267 |
| Rwork | 0.221 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.672 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (8.0.006) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.320 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.104 | |
| Rpim | 0.066 | 0.133 |
| Number of reflections | 16717 | 2738 |
| <I/σ(I)> | 11.5 | 5.7 |
| Completeness [%] | 97.5 | 99.8 |
| Redundancy | 4.8 | 5.6 |
| CC(1/2) | 0.986 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 35% PEG 4000 |
