9BON
Crystal structure of glucosyltransferase (GTD) domain of TpeL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.093, 64.730, 121.181 |
Unit cell angles | 90.00, 102.03, 90.00 |
Refinement procedure
Resolution | 73.445 - 2.222 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.008 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.520 | 10.310 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.080 | |
Rmeas | 0.090 | |
Rpim | 0.042 | |
Number of reflections | 56042 | 2780 |
<I/σ(I)> | 11.2 | 2.1 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 4.6 | |
CC(1/2) | 0.997 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M potassium sodium tartrate and 20% (w/v) PEG 3350 |