9BNA
RE-REFINEMENT OF THE B-DODECAMER D(CGCGAATTCGCG) WITH A COMPARATIVE ANALYSIS OF THE SOLVENT IN IT AND IN THE Z-HEXAMER D(5BRCG5BRCG5BRCG)
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.870, 40.390, 66.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.900 |
| R-factor | 0.188 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.026 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 5691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 * | 290 | Wing, R., (1980) Nature, 287, 755. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE_HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | ||
| 2 | 1 | drop | magnesium acetate | ||
| 3 | 1 | drop | spermine hydrochloride molecule | ||
| 4 | 1 | reservoir | MPD | 20-30 (%) |
