9BKB
Crystal structure of Rid family protein ACIAD3089 from Acinetobacter baylyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-09 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.410, 87.410, 183.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.280 - 2.100 |
| R-factor | 0.1997 |
| Rwork | 0.199 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.939 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.280 | 2.400 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.074 | 0.558 |
| Number of reflections | 68037 | 2648 |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 94.2 | |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.2M sodium sulfate, Reductive methylation |
