9BKA
Crystal structure of Rid family protein PSPTO3006
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-23 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 71.307, 71.307, 71.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.170 - 1.860 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.19840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.841 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.069 | 0.316 |
| Number of reflections | 10209 | 384 |
| <I/σ(I)> | 31.79 | 2.23 |
| Completeness [%] | 98.1 | |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.8 M Potassium sodium tartrate tetrahydrate,0.1 M Tris pH 8.5,0.5% w/v Polyethylene glycol monomethyl ether 5,000 |
