9BEU

Structure of GH110B in complex with a lambda-carrageenan oligosaccharide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541
Spacegroup name	C 1 2 1
Unit cell lengths	168.463, 128.350, 98.905
Unit cell angles	90.00, 122.15, 90.00

Refinement procedure

Resolution	29.963 - 2.400
R-factor	0.1993
Rwork	0.197
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.117	0.479
Rpim	0.059	0.332
Number of reflections	69196	3433
<I/σ(I)>	11.5
Completeness [%]	99.8
Redundancy	4.7
CC(1/2)	0.993	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	18 % PEG 3350, 0.725 M NaI, 2% Tacsimate, 2.5% glycerol, 0.1 M HEPES (pH 7.5)