9B9L

RPRD1B C-terminal interacting domain bound to a pThr4 CTD peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	66.317, 90.231, 135.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.790 - 2.500
R-factor	0.2737
Rwork	0.266
R-free	0.34700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.314
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge		0.051	0.494
Rmeas		0.059	0.562
Rpim		0.030	0.260
Number of reflections	13291	775	407
<I/σ(I)>	5.1
Completeness [%]	92.1	97	56.4
Redundancy	3.9	3.8	3.7
CC(1/2)	0.930	0.996	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Co-crystallization of CID with CTD peptide occurred in a reservoir solution of 20-32% PEG3350 and 0.1M lithium sulfate. 1:3 molar ratio of protein to peptide was used