9B8U
Crystal structure of CRX-Ret4 oligonucleotide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-27 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.597, 75.494, 97.229 |
Unit cell angles | 90.00, 120.25, 90.00 |
Refinement procedure
Resolution | 54.670 - 2.900 |
R-factor | 0.253 |
Rwork | 0.249 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.563 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.400 | 3.120 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.265 | 0.976 |
Rmeas | 0.289 | 1.051 |
Rpim | 0.112 | 0.389 |
Number of reflections | 15350 | 2466 |
<I/σ(I)> | 9.5 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 14.2 |
CC(1/2) | 0.952 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 80 mM KCl, 40 mM cacodylate pH 6.0, 55% MPD, 12 mM Spermine tetrahydrochloride |