9B8U

Crystal structure of CRX-Ret4 oligonucleotide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	109.597, 75.494, 97.229
Unit cell angles	90.00, 120.25, 90.00

Refinement procedure

Resolution	54.670 - 2.900
R-factor	0.253
Rwork	0.249
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.400	3.120
High resolution limit [Å]	2.900	2.900
Rmerge	0.265	0.976
Rmeas	0.289	1.051
Rpim	0.112	0.389
Number of reflections	15350	2466
<I/σ(I)>	9.5	3.4
Completeness [%]	100.0	100
Redundancy	13.4	14.2
CC(1/2)	0.952	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	80 mM KCl, 40 mM cacodylate pH 6.0, 55% MPD, 12 mM Spermine tetrahydrochloride