9B4P
Tetramer Formation of the BCL11A ZF0 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2022-04-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.404, 58.311, 70.087 |
| Unit cell angles | 90.00, 134.12, 90.00 |
Refinement procedure
| Resolution | 50.310 - 2.560 |
| R-factor | 0.2789 |
| Rwork | 0.278 |
| R-free | 0.29410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.310 | 2.649 |
| High resolution limit [Å] | 2.558 | 2.558 |
| Rmerge | 0.141 | 0.680 |
| Rmeas | 0.169 | 0.807 |
| Rpim | 0.091 | 0.431 |
| Number of reflections | 31144 | 955 |
| <I/σ(I)> | 5.28 | 1.19 |
| Completeness [%] | 97.3 | 98.56 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.985 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.3 | 291.15 | 0.2M potassium nitrate, 0.1M Tris-HCl pH 7.3, 20% PEG3350 |
