9B44
Crystal structure of mAb 8-24 Fab, a VRC01-like HIV-1 antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00004 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.874, 77.699, 266.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.360 - 2.010 |
R-factor | 0.2299 |
Rwork | 0.227 |
R-free | 0.28060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.937 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.360 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.110 | 0.604 |
Rmeas | 0.121 | 0.672 |
Rpim | 0.049 | 0.288 |
Total number of observations | 340358 | 21759 |
Number of reflections | 58632 | 4214 |
<I/σ(I)> | 10 | 2.4 |
Completeness [%] | 99.1 | |
Redundancy | 5.8 | 5.2 |
CC(1/2) | 0.996 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG3350, 0.4M KSCN |