9AYI
Human malic enzyme 2 cofactor complex at 1.89 Angstrom.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.325, 98.166, 107.739 |
| Unit cell angles | 65.32, 70.75, 74.89 |
Refinement procedure
| Resolution | 47.420 - 1.890 |
| R-factor | 0.17836 |
| Rwork | 0.177 |
| R-free | 0.20096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.420 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 200119 | 8176 |
| <I/σ(I)> | 6.9 | 1.6 |
| Completeness [%] | 98.2 | 79.9 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.990 | 0.483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M Bis-Tris, 0.1 M Ammonium Tartrate, 0.02 M Potassium Citrate, 20% PEG-3350. |
