9AY8
Structure of the A type blood alpha-D-galactosamine galactosaminidase from Flavonifractor plautii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1806 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.685, 164.568, 131.645 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 39.440 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.465 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.440 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 1.244 |
| Rmeas | 0.075 | 1.472 |
| Rpim | 0.040 | 0.781 |
| Total number of observations | 48915 | |
| Number of reflections | 290235 | 14258 |
| <I/σ(I)> | 13.6 | 1 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.998 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M BICINE pH 9, 20% w/v PEG 6000 |
