9AXQ

Crystal Structure of HY11-7E1_Hu3 Fab in the Apo Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-27
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	72.581, 151.103, 104.604
Unit cell angles	90.00, 91.14, 90.00

Refinement procedure

Resolution	42.830 - 2.200
R-factor	0.2091
Rwork	0.208
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.132	0.101	0.876
Rmeas	0.161	0.122	1.134
Rpim	0.091	0.068	0.711
Total number of observations	284536
Number of reflections	111677	5856	4405
<I/σ(I)>	7.4
Completeness [%]	95.7	98.3	76.1
Redundancy	2.5	3	1.9
CC(1/2)	0.978	0.980	0.417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M ammonium sulfate, 0.1M sodium acetate pH 4.6, 25% PEG 4K