9AXH
Crystal structure of KSR1/MEK1 complex heterotetramer with NST-628
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-06 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97629 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 249.870, 67.080, 74.400 |
Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
Resolution | 52.240 - 2.810 |
R-factor | 0.2057 |
Rwork | 0.203 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.627 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.240 | 2.910 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.051 | 0.670 |
Number of reflections | 30392 | 2553 |
<I/σ(I)> | 18 | |
Completeness [%] | 99.9 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M SPG buffer pH 7.0, 25% w/v PEG 1500 |