9AWE

The crystal structure of an engineered Protein GF with Human Kappa Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 2 21 21
Unit cell lengths	57.869, 85.660, 180.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.050 - 2.800
R-factor	0.2526
Rwork	0.250
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.209
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.050	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	21810	2203
<I/σ(I)>	7.7
Completeness [%]	97.4
Redundancy	1.9
CC(1/2)	0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Sodium Cacodylate pH 6.0, 0.1M Calcium acetate hydrate, 10% PEG 8000