9AVN

Crystal Structure of CARD9 coiled-coil K156-K214 bound to Compound 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	56.390, 56.050, 84.300
Unit cell angles	90.00, 97.75, 90.00

Refinement procedure

Resolution	83.530 - 2.726
R-factor	0.2756
Rwork	0.274
R-free	0.29880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.530	83.530	2.800
High resolution limit [Å]	2.726	12.190	2.730
Rmerge	0.070	0.042	0.507
Rmeas	0.097	0.059	0.695
Number of reflections	25922	285	2007
<I/σ(I)>	6.64
Completeness [%]	95.0
Redundancy	1.8
CC(1/2)	0.995	0.993	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	8-14% PEG 3350