9AUS

Crystal structure of loop-closed dumbbell RNA bridged by glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	P 1
Unit cell lengths	38.514, 82.085, 92.668
Unit cell angles	63.95, 88.69, 88.40

Refinement procedure

Resolution	46.070 - 2.070
R-factor	0.2222
Rwork	0.221
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	MOSFLM (1.20.1_4487)
Data scaling software	SCALEPACK
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.070	2.144
High resolution limit [Å]	2.070	2.070
Number of reflections	57272	5776
<I/σ(I)>	6.55
Completeness [%]	91.1
Redundancy	3.9
CC(1/2)	0.992	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	277.15	0.2 M Lithium sulfate monohydrate, 20% w/v Polyethylene glycol 3,350,