Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.616, 92.616, 105.043 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.370 - 1.230 |
| R-factor | 0.16747 |
| Rwork | 0.167 |
| R-free | 0.17804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.924 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.370 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.118 | 0.950 |
| Rmeas | 0.136 | 1.097 |
| Rpim | 0.066 | 0.543 |
| Number of reflections | 150636 | 7387 |
| <I/σ(I)> | 6.4 | 1.3 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 4 | 4 |
| CC(1/2) | 0.984 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08 M MES-NaOH pH 6.0, 16%(w/v) PEG6000, and 0.16 M NaCl |
