8ZX2
Structure-Based Mechanism and Specificity of Human Galactosyltransferase B3GalT5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-22 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.597, 86.496, 86.219 |
| Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
| Resolution | 27.330 - 2.400 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.148 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.067 | 0.724 |
| Number of reflections | 24939 | 1763 |
| <I/σ(I)> | 21.76 | 2.15 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M BIS-TRIS Propane pH8.5, 0.2M Potassium Sodium Tartrate, 20% w/v PEG 3350 |
