8ZVM
Human citrate synthase intermediate 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.213, 59.819, 73.241 |
| Unit cell angles | 101.72, 98.66, 116.55 |
Refinement procedure
| Resolution | 51.000 - 2.270 |
| R-factor | 0.22145 |
| Rwork | 0.219 |
| R-free | 0.26839 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.940 | 2.170 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.115 | 0.603 |
| Rmeas | 0.149 | 0.793 |
| Number of reflections | 35465 | 1773 |
| <I/σ(I)> | 2.9 | 1.5 |
| Completeness [%] | 84.6 | |
| Redundancy | 2 | |
| CC(1/2) | 0.981 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium acetate pH=7.1, 20% w/v PEG3350 |
