8ZVD
ShosT with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 289 |
| Detector technology | PIXEL |
| Collection date | 2023-07-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.848, 64.288, 64.401 |
| Unit cell angles | 90.00, 90.58, 90.00 |
Refinement procedure
| Resolution | 28.790 - 1.720 |
| R-factor | 0.1599 |
| Rwork | 0.159 |
| R-free | 0.19360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 43288 | 2123 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.990 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 6000, 0.1M MES pH6.2 |
