8ZVB

The ShosTA toxin-antitoxin system provides phage defence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	49.381, 64.736, 64.473
Unit cell angles	90.00, 91.12, 90.00

Refinement procedure

Resolution	39.570 - 2.040
R-factor	0.1936
Rwork	0.192
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.590
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.040	2.090
Number of reflections	25046	1195
<I/σ(I)>	8.7
Completeness [%]	98.4
Redundancy	5.7
CC(1/2)	0.966	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	20%PEG 6000, 0.1M MES pH6.2