8ZUH

Crystal structure of bovine Fbs2/Skp1/Man3GlcNAc2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9000
Spacegroup name	P 41
Unit cell lengths	115.720, 115.720, 110.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.910 - 3.200
R-factor	0.2084
Rwork	0.206
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q3SX24-F1 2e31
RMSD bond length	0.010
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.174	1.244
Number of reflections	24111	3869
<I/σ(I)>	10.4	2
Completeness [%]	99.6	99.8
Redundancy	7	6.8
CC(1/2)	0.995	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.9 M Sodium citrate/0.1 M Tris-HCl (pH 8.0)