8ZSW

Crystal Structure of Human DDB1, a Component of the E3 Ubiquitin Ligase Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	97.590, 118.310, 120.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.730 - 2.250
R-factor	0.1905
Rwork	0.188
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.730	2.330
High resolution limit [Å]	2.250	2.250
Rpim	0.026	0.314
Number of reflections	66888	6617
<I/σ(I)>	17.24	2.88
Completeness [%]	99.7	99.91
Redundancy	12.8	12.3
CC(1/2)	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	287	0.1M MES (pH 6.5), 0.1M sodium acetate, 27%(w/v) PEG 400