8ZQA

Crystal structure of 1,4-alpha-glucan branching protein from Rhodothermus profundi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	100.302, 112.266, 166.260
Unit cell angles	73.59, 73.77, 65.25

Refinement procedure

Resolution	100.100 - 2.910
R-factor	0.2
Rwork	0.197
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.100	3.060
High resolution limit [Å]	2.910	2.910
Number of reflections	135474	19738
<I/σ(I)>	3.6
Completeness [%]	99.0
Redundancy	3.5	3.5
CC(1/2)	0.933	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1M MES, PEG1000, PEG3350, MPD,