8ZO0

Samll GTPase RhoA Y42C mutant in complex with GTP analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97867
Spacegroup name	P 21 21 21
Unit cell lengths	32.278, 63.682, 81.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.350 - 2.210
Rwork	0.184
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
RMSD bond length	0.006
RMSD bond angle	0.851
Data scaling software	HKL-3000
Phasing software	PHENIX ((1.21.1_5286))
Refinement software	PHENIX ((1.21.1_5286))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	2.290
High resolution limit [Å]	2.210	2.210
Rmerge	0.001	0.000
Number of reflections	8444	458
<I/σ(I)>	24.4
Completeness [%]	95.2
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Bis-Tris (pH 5.5), 25% PEG3350