8ZNY

Small GTPase RhoA Y42C mutant in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	32.140, 64.060, 82.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 1.650
Rwork	0.183
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
RMSD bond length	0.006
RMSD bond angle	0.903
Refinement software	PHENIX ((1.21.1_5286))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.140	1.690
High resolution limit [Å]	1.650	1.650
Rmerge	0.089	1.556
Number of reflections	21218	1555
<I/σ(I)>	16.6
Completeness [%]	100.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Ammonium acetate, o.1 M Sodium acetate trihydrate (pH 4.6), 30% PEG4000