8ZNW
Crystal structure of beta-glucosidase Cba3 from Cellulomonas biazotea in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.080, 72.354, 78.381 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 49.060 - 1.900 |
| R-factor | 0.15614 |
| Rwork | 0.154 |
| R-free | 0.18783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 35911 | 1755 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 98.6 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.964 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Na-cacodylate, PEG 8000, calcium acetate |
