8ZNT
Catalytic antibody T99_C220A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.085, 85.326, 104.580 |
| Unit cell angles | 106.77, 91.44, 116.91 |
Refinement procedure
| Resolution | 28.110 - 2.610 |
| R-factor | 0.1974 |
| Rwork | 0.195 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hcr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.444 |
| Number of reflections | 66456 | 3335 |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 3.9 | |
| CC(1/2) | 0.983 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 14%(w/v) PG3350, 0.4 M KSCN, 0.1 M HEPES(pH7.5), 30%(v/v) Glycerol |
