8ZNK

chromone glycosyltransferase (UGT93BB1,SdUGT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-24
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	80.560, 67.610, 93.850
Unit cell angles	90.00, 99.40, 90.00

Refinement procedure

Resolution	34.530 - 1.880
R-factor	0.1889
Rwork	0.187
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.127
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.530	1.930
High resolution limit [Å]	1.880	1.880
Rmerge	0.122	1.379
Rmeas	0.132	1.492
Rpim	0.050	0.566
Total number of observations	555217	39877
Number of reflections	80838	5848
<I/σ(I)>	12.7	1.6
Completeness [%]	99.7
Redundancy	6.9	6.8
CC(1/2)	0.998	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.1M HEPES, 27% 3350, pH 7.0