8ZN7
Crystal Structure of Designed Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.977, 175.183, 94.774 |
| Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.540 |
| R-factor | 0.22708 |
| Rwork | 0.225 |
| R-free | 0.27249 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.090 | 0.710 |
| Number of reflections | 98128 | 9618 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 313 | Tris-HCl KCl Sodium malonate |
