8ZN6
Crystal Structure of Designed Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.760, 110.270, 166.890 |
| Unit cell angles | 77.97, 87.25, 82.41 |
Refinement procedure
| Resolution | 48.740 - 2.940 |
| R-factor | 0.24189 |
| Rwork | 0.239 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.639 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 3.050 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 132294 | 12958 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 313 | Sodium formate, Sodium malonate |
