8ZMX

Crystal Structures of meso-diaminopimelate dehydrogenase from Bacillus thermozeamaize

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	157.090, 157.090, 111.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.810 - 2.460
R-factor	0.2275
Rwork	0.225
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbb
RMSD bond length	0.009
RMSD bond angle	1.089
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.810	2.548
High resolution limit [Å]	2.460	2.460
Rmerge	0.219	5.182
Number of reflections	51060	5017
<I/σ(I)>	16.38
Completeness [%]	99.9
Redundancy	26.8
CC(1/2)	0.999	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M Carboxylic acids; 0.1 M Imidazole-MES monohydrate (acid) pH 6.5; 20% v/v Ethylene glycol; 10% w/v PEG 8000