8ZLW

Crystal Structure of RDGC IQ motif/dCaM Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	52.664, 61.590, 88.002
Unit cell angles	90.00, 94.17, 90.00

Refinement procedure

Resolution	29.260 - 2.200
R-factor	0.218
Rwork	0.216
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.949
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.260	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.163	0.554
Number of reflections	28328	28328
<I/σ(I)>	8.1
Completeness [%]	98.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2 % v/v Tacsimate pH 4.0, 0.1 M Sodium acetate trihydrate pH 4.6, 16 % w/v PEG 3350